1.基本信息
质控结果
| Sample |
sample |
| Read长度 |
100:100 |
| 原始Read数 |
60,000,000 |
| Clean Read数 |
59,207,022 |
| Clean Read比例 |
98.68% |
| 原始Read Q20 |
94.07% |
| 原始Read1 Q20 |
97.85% |
| 原始Read2 Q20 |
90.29% |
| 原始Read Q30 |
84.31% |
| 原始Read1 Q30 |
91.39% |
| 原始Read2 Q30 |
77.23% |
| 原始Read GC比例 |
47.48% |
| Clean Read Q20 |
94.16% |
| Clean Read1 Q20 |
97.89% |
| Clean Read2 Q20 |
90.44% |
| Clean Read Q30 |
84.41% |
| Clean Read1 Q30 |
91.44% |
| Clean Read2 Q30 |
77.38% |
| Clean Read GC比例 |
47.58% |
| Read1 AT分离比例 |
0.10% |
| Read2 AT分离比例 |
0.90% |
| Read1 GC分离比例 |
0.60% |
| Read2 GC分离比例 |
0.80% |
碱基分布
碱基质量分布
2.比对信息
比对结果
| Sample |
sample |
| 比对率 |
99.79% |
| PE比对率 |
99.73% |
| 唯一序列比例 |
89.93% |
| 重复序列比例 |
4.79% |
| 错配率 |
0.77% |
| 嵌合率 |
0.38% |
| 插入片段 |
378.3 |
| Read水平捕获率 |
60.04% |
| 碱基水平捕获率 |
49.97% |
| 平均测序深度(去除重复) |
47.44 |
| 覆盖度(>=1X) |
99.40% |
| 覆盖度(>=2X) |
98.94% |
| 覆盖度(>=3X) |
98.41% |
| 覆盖度(>=4X) |
97.78% |
| 覆盖度(>=5X) |
97.06% |
| 覆盖度(>=10X) |
92.16% |
| 覆盖度(>=20X) |
77.36% |
| 覆盖度(>=30X) |
60.52% |
| 覆盖度(>=100X) |
8.21% |
| 覆盖度均一性(>0.2f) |
92.16% |
| FOLD_80_BASE_PENALTY |
2.742 |
插入片段
深度分布
3.变异检测
变异统计
| Sample |
sample |
| SNP个数 |
49,971 |
| dbSNP比例 |
99.78% |
| 未知SNP |
112 |
| 未知SNP比例 |
0.22% |
| Ti/Tv |
2.48 |
| INDEL个数 |
6,001 |
| dbINDEL比例 |
98.2% |
